Skip to main content
Holiday Schedule: Addgene will be closed November 24th & 25th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCEP4-myc-ACE2 Citations (20)

Plasmid Article: Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2.
Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert AS, Procko E Science. 2020 Sep 4;369(6508):1261-1265. doi: 10.1126/science.abc0870. Epub 2020 Aug 4.
PubMed Journal

Articles Citing pCEP4-myc-ACE2

Articles
The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2. Procko E. bioRxiv. 2020 May 11. doi: 10.1101/2020.03.16.994236. PubMed
Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor. Suh JS, Kim HS, Kim TJ. Sens Actuators B Chem. 2021 May 1;334:129663. doi: 10.1016/j.snb.2021.129663. Epub 2021 Feb 16. PubMed
Experimental Evidence for Enhanced Receptor Binding by Rapidly Spreading SARS-CoV-2 Variants. Laffeber C, de Koning K, Kanaar R, Lebbink JHG. J Mol Biol. 2021 Jul 23;433(15):167058. doi: 10.1016/j.jmb.2021.167058. Epub 2021 May 21. PubMed
Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion. Kim TY, Jeon S, Jang Y, Gotina L, Won J, Ju YH, Kim S, Jang MW, Won W, Park MG, Pae AN, Han S, Kim S, Lee CJ. Exp Mol Med. 2021 May;53(5):956-972. doi: 10.1038/s12276-021-00624-9. Epub 2021 May 25. PubMed
ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion. Zhang Q, Gefter J, Sneddon WB, Mamonova T, Friedman PA. iScience. 2021 Jul 23;24(7):102770. doi: 10.1016/j.isci.2021.102770. Epub 2021 Jun 24. PubMed
SARS-COV-2 spike binding to ACE2 in living cells monitored by TR-FRET. Cecon E, Burridge M, Cao L, Carter L, Ravichandran R, Dam J, Jockers R. Cell Chem Biol. 2021 Jul 2. pii: S2451-9456(21)00307-X. doi: 10.1016/j.chembiol.2021.06.008. PubMed

Associated Plasmids

GB-2 blocking the interaction between ACE2 and wild type and mutation of spike protein of SARS-CoV-2. Tsai MS, Yang YH, Lin YS, Chang GH, Hsu CM, Yeh RA, Shu LH, Cheng YC, Liu HT, Wu YH, Wu YH, Shen RC, Wu CY. Biomed Pharmacother. 2021 Oct;142:112011. doi: 10.1016/j.biopha.2021.112011. Epub 2021 Aug 5. PubMed
Computationally Designed ACE2 Decoy Receptor Binds SARS-CoV-2 Spike (S) Protein with Tight Nanomolar Affinity. Havranek B, Chan KK, Wu A, Procko E, Islam SM. J Chem Inf Model. 2021 Sep 27;61(9):4656-4669. doi: 10.1021/acs.jcim.1c00783. Epub 2021 Aug 24. PubMed
Instant determination of the artemisinin from various Artemisia annua L. extracts by LC-ESI-MS/MS and their in-silico modelling and in vitro antiviral activity studies against SARS-CoV-2. Dogan K, Erol E, Didem Orhan M, Degirmenci Z, Kan T, Gungor A, Yasa B, Avsar T, Cetin Y, Durdagi S, Guzel M. Phytochem Anal. 2022 Mar;33(2):303-319. doi: 10.1002/pca.3088. Epub 2021 Sep 28. PubMed
The neutralization effect of montelukast on SARS-CoV-2 is shown by multiscale in silico simulations and combined in vitro studies. Durdagi S, Avsar T, Orhan MD, Serhatli M, Balcioglu BK, Ozturk HU, Kayabolen A, Cetin Y, Aydinlik S, Bagci-Onder T, Tekin S, Demirci H, Guzel M, Akdemir A, Calis S, Oktay L, Tolu I, Butun YE, Erdemoglu E, Olkan A, Tokay N, Isik S, Ozcan A, Acar E, Buyukkilic S, Yumak Y. Mol Ther. 2022 Feb 2;30(2):963-974. doi: 10.1016/j.ymthe.2021.10.014. Epub 2021 Oct 19. PubMed
Disulfide Bonds Play a Critical Role in the Structure and Function of the Receptor-binding Domain of the SARS-CoV-2 Spike Antigen. Grishin AM, Dolgova NV, Landreth S, Fisette O, Pickering IJ, George GN, Falzarano D, Cygler M. J Mol Biol. 2022 Jan 30;434(2):167357. doi: 10.1016/j.jmb.2021.167357. Epub 2021 Nov 12. PubMed
COVID-19 Specific Immune Markers Revealed by Single Cell Phenotypic Profiling. Sansico F, Miroballo M, Bianco DS, Tamiro F, Colucci M, Santis E, Rossi G, Rosati J, Di Mauro L, Miscio G, Mazza T, Vescovi AL, Mazzoccoli G, Giambra V, On Behalf Of Css-Covid Group. Biomedicines. 2021 Nov 29;9(12). pii: biomedicines9121794. doi: 10.3390/biomedicines9121794. PubMed
Engineered High-Affinity ACE2 Peptide Mitigates ARDS and Death Induced by Multiple SARS-CoV-2 Variants. Zhang L, Dutta S, Xiong S, Chan M, Chan KK, Fan TM, Bailey KL, Lindeblad M, Cooper LM, Rong L, Gugliuzza AF, Shukla D, Procko E, Rehman J, Malik AB. bioRxiv. 2021 Dec 22. doi: 10.1101/2021.12.21.473668. PubMed
Glycoprotein Targeted CAR-NK Cells for the Treatment of SARS-CoV-2 Infection. Christodoulou I, Rahnama R, Ravich JW, Seo J, Zolov SN, Marple AN, Markovitz DM, Bonifant CL. Front Immunol. 2021 Dec 23;12:763460. doi: 10.3389/fimmu.2021.763460. eCollection 2021. PubMed
Engineered ACE2 decoy mitigates lung injury and death induced by SARS-CoV-2 variants. Zhang L, Dutta S, Xiong S, Chan M, Chan KK, Fan TM, Bailey KL, Lindeblad M, Cooper LM, Rong L, Gugliuzza AF, Shukla D, Procko E, Rehman J, Malik AB. Nat Chem Biol. 2022 Mar;18(3):342-351. doi: 10.1038/s41589-021-00965-6. Epub 2022 Jan 19. PubMed
Loss of Detection of sgN Precedes Viral Abridged Replication in COVID-19-Affected Patients-A Target for SARS-CoV-2 Propagation. Ferrucci V, de Antonellis P, Quarantelli F, Asadzadeh F, Bibbo F, Siciliano R, Sorice C, Pisano I, Izzo B, Di Domenico C, Boccia A, Vargas M, Pierri B, Viscardi M, Brandi S, Fusco G, Cerino P, De Pietro L, Furfaro C, Napolitano LA, Paolella G, Festa L, Marzinotto S, Conte MC, Gentile I, Servillo G, Curcio F, de Cristofaro T, Broccolo F, Capoluongo E, Zollo M. Int J Mol Sci. 2022 Feb 9;23(4). pii: ijms23041941. doi: 10.3390/ijms23041941. PubMed
Potential inhibitor for blocking binding between ACE2 and SARS-CoV-2 spike protein with mutations. Tsai MS, Shih WT, Yang YH, Lin YS, Chang GH, Hsu CM, Yeh RA, Shu LH, Cheng YC, Liu HT, Wu YH, Wu YH, Shen RC, Wu CY. Biomed Pharmacother. 2022 May;149:112802. doi: 10.1016/j.biopha.2022.112802. Epub 2022 Mar 9. PubMed
Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium. Pinto AL, Rai RK, Brown JC, Griffin P, Edgar JR, Shah A, Singanayagam A, Hogg C, Barclay WS, Futter CE, Burgoyne T. Nat Commun. 2022 Mar 25;13(1):1609. doi: 10.1038/s41467-022-29255-y. PubMed
Induction of SARS-CoV-2 neutralizing antibodies by CoronaVac and BNT162b2 vaccines in naive and previously infected individuals. Muena NA, Garcia-Salum T, Pardo-Roa C, Avendano MJ, Serrano EF, Levican J, Almonacid LI, Valenzuela G, Poblete E, Strohmeier S, Salinas E, Munoz A, Haslwanter D, Dieterle ME, Jangra RK, Chandran K, Gonzalez C, Riquelme A, Krammer F, Tischler ND, Medina RA. EBioMedicine. 2022 Apr;78:103972. doi: 10.1016/j.ebiom.2022.103972. Epub 2022 Mar 30. PubMed
Caveolin-1-ACE2 axis modulates xenobiotic metabolism-linked chemoresistance in ovarian clear cell carcinoma. Nagappan A, Kim KH, Moon Y. Cell Biol Toxicol. 2022 May 27. pii: 10.1007/s10565-022-09733-1. doi: 10.1007/s10565-022-09733-1. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.