|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||141253||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGari et al., 1997/DiCarlo et al., 2013
Modifications to backboneReplace CEN/ARS with 2µ; Replace TEF1 promoter with GAL1
Vector typeYeast Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAS9i_TRP was a gift from Adam Bertl (Addgene plasmid # 141253 ; http://n2t.net/addgene:141253 ; RRID:Addgene_141253)