pLD-puro-Cc-TARDBP-G368A_VA
(Plasmid
#141330)
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PurposeLentiviral vector for expression of C-terminally VA-tagged human TARDBP-G368A in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLD-puro-Cc-VA (MAPLE vector)
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Backbone manufacturerAddgene# 24588
- Backbone size w/o insert (bp) 9389
- Total vector size (bp) 8983
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsGrow for no longer than 16 hr
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTARDBP-G368A
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SpeciesH. sapiens (human)
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Insert Size (bp)1608
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MutationG368A
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GenBank ID23435
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Entrez GeneTARDBP (a.k.a. ALS10, TDP-43)
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Tag
/ Fusion Protein
- VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG) (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer 5'-TCTTTCCCCTGCACTGTACC-3' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLD-puro-Cc-TARDBP-G368A_VA was a gift from Mohan Babu (Addgene plasmid # 141330 ; http://n2t.net/addgene:141330 ; RRID:Addgene_141330) -
For your References section:
BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain. Pourhaghighi R, Ash PEA, Phanse S, Goebels F, Hu LZM, Chen S, Zhang Y, Wierbowski SD, Boudeau S, Moutaoufik MT, Malty RH, Malolepsza E, Tsafou K, Nathan A, Cromar G, Guo H, Abdullatif AA, Apicco DJ, Becker LA, Gitler AD, Pulst SM, Youssef A, Hekman R, Havugimana PC, White CA, Blum BC, Ratti A, Bryant CD, Parkinson J, Lage K, Babu M, Yu H, Bader GD, Wolozin B, Emili A. Cell Syst. 2020 Apr 22;10(4):333-350.e14. doi: 10.1016/j.cels.2020.03.003. 10.1016/j.cels.2020.03.003 PubMed 32325033
