|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1414||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5100
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2050
Mutationdeleted amino acids 377 to 415
Entrez GeneGATA2 (a.k.a. DCML, IMD21, MONOMAC, NFE1B)
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (not destroyed)
- 3′ cloning site SnaBI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabeHygro-GATA2-D377-415 was a gift from Gokhan Hotamisligil (Addgene plasmid # 1414 ; http://n2t.net/addgene:1414 ; RRID:Addgene_1414)
For your References section:Interaction between GATA and the C/EBP family of transcription factors is critical in GATA-mediated suppression of adipocyte differentiation. Tong Q, Tsai J, Tan G, Dalgin G, Hotamisligil GS. Mol Cell Biol 2005 Jan;25(2):706-15. 10.1128/MCB.25.2.706-715.2005 PubMed 15632071