pMBP-HRV3C-AcrIF9
(Plasmid
#141442)
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PurposeExpression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141442 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMAL-c4x
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Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 6645
- Total vector size (bp) 6850
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAcrIF9
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Alt nameAcrF9
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Alt nameF9
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SpeciesProteus penneri ATCC 35198
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Insert Size (bp)207
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GenBank IDEEG86164.1
- Promoter ptac
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Tags
/ Fusion Proteins
- Maltose Binding Protein (N terminal on backbone)
- HRV3C cleavage site (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ptac (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMBP-HRV3C-AcrIF9 was a gift from Blake Wiedenheft (Addgene plasmid # 141442 ; http://n2t.net/addgene:141442 ; RRID:Addgene_141442) -
For your References section:
AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex. Hirschi M, Lu WT, Santiago-Frangos A, Wilkinson R, Golden SM, Davidson AR, Lander GC, Wiedenheft B. Nat Commun. 2020 Jun 1;11(1):2730. doi: 10.1038/s41467-020-16512-1. 10.1038/s41467-020-16512-1 PubMed 32483187
Map uploaded by the depositor.
