|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14230||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression ; Gateway Destination
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsDB3.1, ccdB Survival
/ Fusion Protein
- 3HA (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer GPDPro_F (Common Sequencing Primers)
Terms and Licenses
Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that this plasmid is missing the Cyc1 terminator. It is unknown how this will affect gene expression.
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAG303GPD-ccdB-HA was a gift from Susan Lindquist (Addgene plasmid # 14230 ; http://n2t.net/addgene:14230 ; RRID:Addgene_14230)