PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||144550||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerBroad Institute Genetic Perturbation Platform
- Backbone size w/o insert (bp) 8264
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)680
Entrez GeneLMO2 (a.k.a. LMO-2, RBTN2, RBTNL1, RHOM2, TTG2)
- Promoter EF1a
/ Fusion Protein
- V5 (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer GGGTGGAGACTGAAGTTAGGCCAG
- 3′ sequencing primer CACATAGCGTAAAAGGAGCAACATAG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
NM_001142315.1 Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TFORF3074 was a gift from Feng Zhang (Addgene plasmid # 144550 ; http://n2t.net/addgene:144550 ; RRID:Addgene_144550)
For your References section:A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell. 2023 Jan 5;186(1):209-229.e26. doi: 10.1016/j.cell.2022.11.026. 10.1016/j.cell.2022.11.026 PubMed 36608654