Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14546||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namePARP1 shRNA
SpeciesH. sapiens (human)
Insert Size (bp)60
Entrez GenePARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer H1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Sense oligo sequence used for cloning: PARP-1 #3, 5'-GATCCCCGGA CTCGCTCCGG ATGGCCTTCA AGAGAGGCCA TCCGGAGCGA GTCCTTTTTG GAAA-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV.PARP1#3 was a gift from Didier Trono (Addgene plasmid # 14546 ; http://n2t.net/addgene:14546 ; RRID:Addgene_14546)
For your References section:DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable for human immunodeficiency virus type 1 integration. Ariumi Y, Turelli P, Masutani M, Trono D. J Virol. 2005 Mar . 79(5):2973-8. 10.1128/JVI.79.5.2973-2978.2005 PubMed 15709017