SG3ΔENV IN D116A
(Plasmid #145786)

• Purpose: Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D116A mutation in integrase (IN D116A).
• Depositing Lab: Wesley Sundquist
• Depositing Organization: University of Utah
  Located in the United States of America
• Publication: Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/6513/eabc8420. doi: 10.1126/science.abc8420.

Backbone

• Vector backbone: pTZ19U
• Backbone manufacturer: Bio-Rad
• Backbone size w/o insert (bp): 2863
• Total vector size (bp): 14788
• Modifications to backbone: none
• Vector type: Mammalian Expression, Lentiviral, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Recombination deficient strain required to avoid recombination of plasmid
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Integrase
• Alt name: IN
• Species: HIV-1
• Mutation: Changed integrase residue aspartic acid 116 to alanine (IN D116A)
• GenBank ID: L02317.1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PfiMI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: CCTGGCATGGGTACCAGCAC
• 3′ sequencing primer: CTAATCCTCATCCTGTCTACT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned from NIH Aids reagent program plasmid SG3delENV catalog #11051

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry