pGEX cycT2b (P#1730)
(Plasmid #14603)

• Depositing Lab: Matija Peterlin
• Publication: Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

Backbone

• Vector backbone: pGEX 4T-1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cycT2
• Alt name: hCycT2
• Alt name: cyclin T2
• Alt name: cyclinT2
• Species: H. sapiens (human)
• Insert Size (bp): 2193
• Entrez Gene: CCNT2 (a.k.a. CYCT2)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: pGEX 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry