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pLINK cyclin T1 (1-280) myc (all 200) (P#575)
(Plasmid #14610)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14610 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 14610-D50 50 μg of DNA in Tris buffer $495
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DNA 14610-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    EF-pLINK2
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cyclin T1 (1-280) (all 200)
  • Alt name
    cyclinT1
  • Alt name
    cycT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    840
  • Mutation
    C198A, C200A, H202A, C205A. Active in 3T3 cells.
  • Entrez Gene
    CCNT1 (a.k.a. CCNT, CYCT1, HIVE1)
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EF1a forward primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expression checked by western blot.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLINK cyclin T1 (1-280) myc (all 200) (P#575) was a gift from Matija Peterlin (Addgene plasmid # 14610 ; http://n2t.net/addgene:14610 ; RRID:Addgene_14610)

  • For your References section:

    Interactions between human cyclin T, Tat, and the transactivation response element (TAR) are disrupted by a cysteine to tyrosine substitution found in mouse cyclin T. Fujinaga K, Taube R, Wimmer J, Cujec TP, Peterlin BM. Proc Natl Acad Sci U S A. 1999 Feb 16. 96(4):1285-90. 10.1073/pnas.96.4.1285 PubMed 9990016
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