pJRL2 PHO5(UASm1)pr VYFP (EB#1549)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14841||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePHO5 promoter UASm1
SpeciesS. cerevisiae (budding yeast)
MutationUASm1 (AAGCTT was substituted for CACGTT). Uninducible promoter. See Steger DJ et al Science 299(5603)114-116.
Entrez GenePHO5 (a.k.a. YBR093C)
/ Fusion Protein
- VYFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJRL2 PHO5(UASm1)pr VYFP (EB#1549) was a gift from Erin O'Shea (Addgene plasmid # 14841 ; http://n2t.net/addgene:14841 ; RRID:Addgene_14841)
For your References section:Control of stochasticity in eukaryotic gene expression. Raser JM, O'Shea EK. Science. 2004 Jun 18. 304(5678):1811-4. 10.1126/science.1098641 PubMed 15166317