pYL192 (TRV1)
(Plasmid #148968)

• Purpose: Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)
• Depositing Lab: Savithramma Dinesh-Kumar
• Publication: Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.1046/j.1365-313x.2002.01297.x.

Backbone

• Vector backbone: pBIN19
• Backbone size w/o insert (bp): 11498
• Total vector size (bp): 19370
• Vector type: Plant vector with TRV RNA1; this vector used with pYL156(TRV RNA2) for virus induced gene silencing (VIGS)
• Selectable markers: Kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: We prefer to use DH10B cells
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TRV RNA1
• Species: Tobacco Rattle Virus RNA 1
• Insert Size (bp): 6791
• Mutation: P697S mutation in putative replicase. S239G and S246P mutation in 16kDa protein.
• GenBank ID: AF406990

Resource Information

• Supplemental Documents:
  • TRV1 and TRV2 VIGS vectors papers
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry