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PurposeExpresses human ACE2 driven by the K18 promoter
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Depositing Lab
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Depositing OrganizationUniversity of Iowa
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149449 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 149449-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 149449-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepSEAP
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Backbone manufacturerTropix, Bedford, MA
- Backbone size w/o insert (bp) 7114
- Total vector size (bp) 9532
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Modifications to backboneThe vector includes 5' and 3' genomic regions of the human K18 gene
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAngiotensin-converting enzyme 2
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SpeciesH. sapiens (human)
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Insert Size (bp)2418
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Entrez GeneACE2 (a.k.a. ACEH)
- Promoter K18
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer -
- 3′ sequencing primer -
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The vector includes 5' and 3' genomic regions of the human K18 gene, necessary for driving high-level epithelial-cell-specific expression
DNA (Catalog # 149449-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 149449-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pK18-hACE2 was a gift from Paul McCray (Addgene plasmid # 149449 ; http://n2t.net/addgene:149449 ; RRID:Addgene_149449) -
For your References section:
Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus. McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M, Manzel L, Shi L, Netland J, Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S. J Virol. 2007 Jan;81(2):813-21. doi: 10.1128/JVI.02012-06. Epub 2006 Nov 1. 10.1128/JVI.02012-06 PubMed 17079315
Map uploaded by the depositor.
