pYPQ166-iSpyMac
(Plasmid
#149553)
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PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYPQ165
- Total vector size (bp) 7751
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Vector typePlant Expression, CRISPR ; Gateway compatible ziSpyMacCas9 entry clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiSpyMacCas9
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Alt name3xFLAG-NLS-ziSpyMacCas9-NLS
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MutationR221K, N394K, Protospacer interacting domain form Streptococcus macacae
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Tags
/ Fusion Proteins
- 3X FLAG, NLS (N terminal on insert)
- NLS (C terminal on insert)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ166-iSpyMac was a gift from Yiping Qi (Addgene plasmid # 149553 ; http://n2t.net/addgene:149553 ; RRID:Addgene_149553) -
For your References section:
Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences. Sretenovic S, Yin D, Levav A, Selengut JD, Mount SM, Qi Y. Plant Commun. 2020 Jul 22;2(2):100101. doi: 10.1016/j.xplc.2020.100101. eCollection 2021 Mar 8. 10.1016/j.xplc.2020.100101 PubMed 33898973
