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pTetO_105
(Plasmid #149599)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 149599 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCFB390
  • Backbone size w/o insert (bp) 6140
  • Vector type
    Yeast Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yeGFP
  • Insert Size (bp)
    717
  • Promoter TEF1-TetO_105

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer gaaattcgcttatttagaagtgtc
  • 3′ sequencing primer CTCCTTCCTTTTCGGTTAGAG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTetO_105 was a gift from Michael Jensen (Addgene plasmid # 149599 ; http://n2t.net/addgene:149599 ; RRID:Addgene_149599)
  • For your References section:

    High-Resolution Scanning of Optimal Biosensor Reporter Promoters in Yeast. Ambri F, D'Ambrosio V, Di Blasi R, Maury J, Jacobsen SAB, McCloskey D, Jensen MK, Keasling JD. ACS Synth Biol. 2020 Feb 21;9(2):218-226. doi: 10.1021/acssynbio.9b00333. Epub 2020 Jan 31. 10.1021/acssynbio.9b00333 PubMed 31935067