|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14960||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1500
Entrez GeneSMAD6 (a.k.a. AOVD2, HsT17432, MADH6, MADH7)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV rev (Common Sequencing Primers)
See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CS2 Smad6 was a gift from Joan Massague (Addgene plasmid # 14960 ; http://n2t.net/addgene:14960 ; RRID:Addgene_14960)
For your References section:Smad6 inhibits BMP/Smad1 signaling by specifically competing with the Smad4 tumor suppressor. Hata A, Lagna G, Massague J, Hemmati-Brivanlou A. Genes Dev. 1998 Jan 15. 12(2):186-97. 10.1101/gad.12.2.186 PubMed 9436979