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pET28(a)-hFX-HA
(Plasmid #14991)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14991 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone manufacturer
    BMD Biosciences
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human frataxin cDNA
  • Alt name
    hFX
  • Alt name
    FRX
  • Alt name
    frataxin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    428
  • Mutation
    contains cDNA coding sequence for amino acids 88-210 of the original human frataxin gene
  • Entrez Gene
    FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • AU1 (C terminal on insert)
    • HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28(a)-hFX-HA was a gift from Michael Ristow (Addgene plasmid # 14991 ; http://n2t.net/addgene:14991 ; RRID:Addgene_14991)
  • For your References section:

    Crystal structure of human frataxin. Dhe-Paganon S, Shigeta R, Chi YI, Ristow M, Shoelson SE. J Biol Chem. 2000 Oct 6. 275(40):30753-6. 10.1074/jbc.C000407200 PubMed 10900192