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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15030 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSP64
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta-globin 5' and 3' UTR
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Alt namebeta-globin
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SpeciesX. laevis (frog)
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Insert Size (bp)260
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Cloning site for mRNA is BglII. See author's map for more information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP64T (DM#111) was a gift from Douglas Melton (Addgene plasmid # 15030 ; http://n2t.net/addgene:15030 ; RRID:Addgene_15030) -
For your References section:
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Krieg PA, Melton DA. Nucleic Acids Res. 1984 Sep 25. 12(18):7057-70. 10.1093/nar/12.18.7057 PubMed 6207484
Map uploaded by the depositor.
