pSP70 Vg1 (DM#139)
(Plasmid
#15070)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15070 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSP70
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVg1
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SpeciesX. laevis (frog)
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Insert Size (bp)2300
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Entrez Genegdf1 (a.k.a. dvr-1, dvr1, gdf-1, vg1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
BamHI to EcoRI fragment of T-Vg1-A. See author's map for additional information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP70 Vg1 (DM#139) was a gift from Douglas Melton (Addgene plasmid # 15070 ; http://n2t.net/addgene:15070 ; RRID:Addgene_15070) -
For your References section:
Localized synthesis of the Vg1 protein during early Xenopus development. Tannahill D, Melton DA. Development. 1989 Aug . 106(4):775-85. PubMed 2562668
Map uploaded by the depositor.
