pBS CHX10 3'UTR (CC#56)
(Plasmid
#15150)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBluescript KS
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCHX10 3'UTR
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SpeciesG. gallus (chicken)
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Insert Size (bp)950
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GenBank IDAF178671
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Entrez GeneVSX2 (a.k.a. CHX10)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Chx10 cDNA fragment containing 0.95 kb of the 3' untranslated region (UTR) (StuI to 3' end). Used for generating antisense riboprobes.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS CHX10 3'UTR (CC#56) was a gift from Connie Cepko (Addgene plasmid # 15150 ; http://n2t.net/addgene:15150 ; RRID:Addgene_15150) -
For your References section:
Expression of Chx10 and Chx10-1 in the developing chicken retina. Chen CM, Cepko CL. Mech Dev. 2000 Feb . 90(2):293-7. 10.1016/S0925-4773(99)00251-8 PubMed 10640715