Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

p2T-CMV-EA-BE4max-NG-BlastR
(Plasmid #152992)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 152992 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p2T-CMV-BlastR
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BE4 H47E+S48A-nSpCas9-UGI-UGI
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2T-CMV-EA-BE4max-NG-BlastR was a gift from David Liu (Addgene plasmid # 152992 ; http://n2t.net/addgene:152992 ; RRID:Addgene_152992)
  • For your References section:

    Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Arbab M, Shen MW, Mok B, Wilson C, Matuszek Z, Cassa CA, Liu DR. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. 10.1016/j.cell.2020.05.037 PubMed 32533916