PurposeExpression construct for producing Avi tagged Lysyl-tRNA synthetase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||153051||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerStructural Genomics Consortium
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
SpeciesH. sapiens (human)
Entrez GeneKARS1 (a.k.a. CMTRIB, DEAPLE, DFNB89, KARS, KARS2, KRS, LEPID)
- Promoter T7
/ Fusion Proteins
- His6-TEV (N terminal on backbone)
- Avi (C terminal on backbone)
- Cloning method Ligation Independent Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Backbone vector pNIC-Bio3, Genbank acc. no. JN792439
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:KARS_pNIC-Bio3 was a gift from Susanne Gräslund (Addgene plasmid # 153051 ; http://n2t.net/addgene:153051 ; RRID:Addgene_153051)