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pLF3FShC
(Plasmid #153083)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 153083 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II SK (+)
  • Backbone manufacturer
    Stratagene Cloning Systems
  • Modifications to backbone
    1) Removal of SapI site in pBluescript II SK (+), 2) Addition of a loxP site, MCS, FRT3 site, FRT site, hygromycin resistance gene, let-858 3' UTR, sqt-1(e1350) gene, hsp-16.41 promoter, Cre recombinase gene, and tbb-2 3' UTR, all via multi-insert Golden Gate Assembly 3) insertion of two SapI sites in MCS.
  • Vector type
    Worm Expression ; Recombination Mediated Cassette Exchange
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Some component parts were obtained from other plasmids. Specifically, the hygR gene, sqt-1(e1350) gene, hsp-16-41 promoter, let-858 and tbb-2 3' UTR were all PCR amplified from Addgene #91829 made by Daniel Dickinson in Bob Goldstein's lab
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLF3FShC was a gift from Michael Nonet (Addgene plasmid # 153083 ; http://n2t.net/addgene:153083 ; RRID:Addgene_153083)
  • For your References section:

    Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Nonet ML. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388. 10.1534/genetics.120.303388 PubMed 32513816