FLAG-SpEAF pNMT-TOPO
(Plasmid #15316)

• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Backbone

• Vector backbone: pNMT-TOPO (modified)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6066
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ELL associated factor
• Alt name: SpEAF
• Alt name: eaf1
• Species: S. pombe (fission yeast)
• Insert Size (bp): 756
• GenBank ID: EF192607
• Entrez Gene: eaf1 (a.k.a. SPCC1223.10c)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: Kpn1 (not destroyed)
• 5′ sequencing primer: NMT forward
• 3′ sequencing primer: URA4 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry