|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||153289||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5121
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameAdenosine sensor GRAB_Ado1.0med (medium affinity)
SpeciesH. sapiens (human)
Insert Size (bp)2256
- Promoter GfaABC1D
- Cloning method Gibson Cloning
Addgene NGS identified a SNP in the WPRE. The depositor does not suspect this is of functional concern but requesting scientists should validate functionality before experimental use. Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.075564v1 for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-GfaABC1D-GRAB_Ado1.0med was a gift from Yulong Li (Addgene plasmid # 153289 ; http://n2t.net/addgene:153289 ; RRID:Addgene_153289)
For your References section:A GRAB sensor reveals activity-dependent non-vesicular somatodendritic adenosine release. Zhaofa Wu, Yuting Cui, Huan Wang, Kun Song, Zhengwei Yuan, Ao Dong, Hao Wu, Yi Wan, Sunlei Pan, Wanling Peng, Miao Jing, Min Xu, Minmin Luo, Yulong Li. bioRxiv 10.1101/2020.05.04.075564