Purposethis is the non-clustering version of LIC-Z tagged with mcherry
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||153545||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namehuman TCR ζ-Chain
SpeciesH. sapiens (human)
Mutationwith Cry2 sequence deleted from LIC-Z
Entrez GeneCD247 (a.k.a. CD3-ZETA, CD3H, CD3Q, CD3Z, CD3ZETA, IMD25, T3Z, TCRZ)
- Promoter CMV
/ Fusion Protein
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LIC-Z-delCry2 was a gift from Katharina Gaus (Addgene plasmid # 153545 ; http://n2t.net/addgene:153545 ; RRID:Addgene_153545)
For your References section:Clustering of the zeta-Chain Can Initiate T Cell Receptor Signaling. Ma Y, Lim YJ, Benda A, Lou J, Goyette J, Gaus K. Int J Mol Sci. 2020 May 15;21(10). pii: ijms21103498. doi: 10.3390/ijms21103498. 10.3390/ijms21103498 PubMed 32429097