Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Donor-CD55-NeoR
(Plasmid #153551)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 153551 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-2Aneo (Addgene #80032)
  • Backbone manufacturer
    Konishi lab
  • Backbone size w/o insert (bp) 4507
  • Total vector size (bp) 5903
  • Modifications to backbone
    Frame Adjuster in pAAV-2Aneo was truncated by digestion with BsrGI followed by self-ligation
  • Vector type
    AAV, Cre/Lox ; Donor plasmid/Targeting vector
  • Selectable markers
    Neomycin (select with G418) ; NeoR/KanR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
  • Alt name
    N/A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1415
  • Mutation
    a 1-bp mutation in exon 4
  • Promoter No promoter
  • Tag / Fusion Protein
    • N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Donor-CD55-NeoR was a gift from Hiroyuki Konishi (Addgene plasmid # 153551 ; http://n2t.net/addgene:153551 ; RRID:Addgene_153551)
  • For your References section:

    Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53. Hyodo T, Rahman ML, Karnan S, Ito T, Toyoda A, Ota A, Wahiduzzaman M, Tsuzuki S, Okada Y, Hosokawa Y, Konishi H. Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064. 10.1016/j.celrep.2019.12.064 PubMed 31995758