His-myc-Med20 pBacPAK8
(Plasmid
#15370)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15370 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBacPAK8
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5538
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Vector typeBaculovirus construction
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMed20
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Alt nameTRFP
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SpeciesN/A
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Insert Size (bp)733
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1/Nhe1 (destroyed during cloning)
- 3′ cloning site Kpn1 (not destroyed)
- 5′ sequencing primer Bac1
- 3′ sequencing primer Bac2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
His-myc-Med20 pBacPAK8 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15370 ; http://n2t.net/addgene:15370 ; RRID:Addgene_15370) -
For your References section:
A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW. J Biol Chem. 2003 Dec 12. 278(50):49671-4. 10.1074/jbc.C300444200 PubMed 14576168