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pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop
(Plasmid #153956)


Item Catalog # Description Quantity Price (USD)
Plasmid 153956 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Login to view industry pricing.


  • Vector backbone
  • Backbone manufacturer
    Rual et al, Genome Res. 14:2128-2135, 2004.
  • Backbone size w/o insert (bp) 5005
  • Vector type
    Gateway-compatible Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    SARS-CoV-2 isolate Wuhan-Hu-1
  • Insert Size (bp)
  • Mutation
    Many synonymous changes due to codon optimization
  • Entrez Gene
    ORF7b (a.k.a. GU280_gp08)

Cloning Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Kim et al. 2020 can be accessed at

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop was a gift from Fritz Roth (Addgene plasmid # 153956 ; ; RRID:Addgene_153956)
  • For your References section:

    A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP. G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. 10.1534/g3.120.401554 PubMed 32763951