PurposeExpress Orf3b gene along with HA and BASU
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||153992||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesSevere acute respiratory syndrome coronavirus 2
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer EF1a-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SARS_COV2_ORF3b_HA_BASU was a gift from Paul Khavari (Addgene plasmid # 153992 ; http://n2t.net/addgene:153992 ; RRID:Addgene_153992)
For your References section:The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted antiviral signaling and host translation. Meyers JM, Ramanathan M, Shanderson RL, Beck A, Donohue L, Ferguson I, Guo MG, Rao DS, Miao W, Reynolds D, Yang X, Zhao Y, Yang YY, Blish C, Wang Y, Khavari PA. PLoS Pathog. 2021 Oct 1;17(10):e1009412. doi: 10.1371/journal.ppat.1009412. 10.1371/journal.ppat.1009412 PubMed 34597346