EC71173
(Plasmid #154061)

• Purpose: Level 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-Cre
• Depositing Lab: Cristobal Uauy
• Publication: Harrington et al Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. eCollection 2020.

Backbone

• Vector backbone: EC47811 (pL1V-R2-47811)
• Backbone manufacturer: Engineering Nitrogen Symbiosis for Africa (ENSA)
• Backbone size w/o insert (bp): 4290
• Total vector size (bp): 6509
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cre recombinase
• Alt name: Cre-U5-Cre
• Species: Enterobacteria phage P1
• Insert Size (bp): 2133
• Mutation: Introduction of U5 intron sequences, to prevent premature activation during cloning.
• GenBank ID: 2777477
• Promoter: HvHSP17

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: GAACCCTGTGGTTGGCATGCACATAC
• 3′ sequencing primer: CTGGTGGCAGGATATATTGTGGTG

Resource Information

• Supplemental Documents:
  • ec71173-pl1m-r2-.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry