EC71139
(Plasmid #154062)

• Purpose: Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)
• Depositing Lab: Cristobal Uauy
• Publication: Harrington et al Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. eCollection 2020.

Backbone

• Vector backbone: pMS (standard backbone from GENEART, Thermofisher)
• Backbone manufacturer: GENEART
• Backbone size w/o insert (bp): 2609
• Total vector size (bp): 4558
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pZmUbi
• Alt name: Zea mays Ubiquitin Promoter
• Species: Zea mays
• Insert Size (bp): 2035
• Mutation: All BsaI, Esp3I and BpiI restriction sites were removed using synonymous changes, flanking BsaI sites were added

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (not destroyed)
• 3′ cloning site: BsaI (not destroyed)
• 5′ sequencing primer: GTAAAACGACGGCCAG

Resource Information

• Supplemental Documents:
  • 13acq2sc_ec71139_pms.gb
• A portion of this plasmid was derived from a plasmid made by: Synthesised by GENEART

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry