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Addgene

pAM552
(Plasmid #154131)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 154131 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Synthetic
  • Backbone size w/o insert (bp) 1836
  • Total vector size (bp) 7452
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    E. coli K12 POP2136
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    wt bacterial rRNAs operon under control of pL promoter
  • Species
    Synthetic
  • Insert Size (bp)
    5615

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM552 was a gift from Alexander Mankin (Addgene plasmid # 154131 ; http://n2t.net/addgene:154131 ; RRID:Addgene_154131)
  • For your References section:

    Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly. Huang S, Aleksashin NA, Loveland AB, Klepacki D, Reier K, Kefi A, Szal T, Remme J, Jaeger L, Vazquez-Laslop N, Korostelev AA, Mankin AS. Nat Commun. 2020 Jun 9;11(1):2900. doi: 10.1038/s41467-020-16694-8. 10.1038/s41467-020-16694-8 PubMed 32518240