pVE2_20_p40S_QfDBD_VP16AD_pFullLength_pcbAB_DsRed
(Plasmid #154242)

• Purpose: Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbAB
• Depositing Lab: Arnold Driessen
• Publication: Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 10.1186/s12934-019-1253-3.

Backbone

• Vector backbone: pAGM4673
• Backbone manufacturer: MoClo Tool Kit of Sylvestre Marillonnet
• Backbone size w/o insert (bp): 8116
• Vector type: Bacterial Expression, Synthetic Biology ; Fungal Expression
• Selectable markers: amdS fungal selection

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p40S:QfDBD-VP16AD-GFP-NLS
• Alt name: P(various):DsRed-SKL
• Alt name: pGpdA:amdS
• Species: Synthetic; Fungal expression, P. rubens (formerly P. chrysogenum)
• Promoter: p40S (AN0465 A. nidulans)
• Tag / Fusion Protein:
  • GFP-NLS; DsRed-SKL (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • graphical abstract.tif
  • pVE2_20_p40S_QfDBD_VP16AD_pFullLength_pcbAB_DsRed.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Synthetic Transcription Factor (QfDBD-VP16AD-GFP-NLS) and DsRed-SKL transcribed with various full and core promoters (CP) with and without Quinic acid Upstream Activation Sequences (QUAS). Fungal selection provided with amdS marker. Flanking regions were designed for integration at the locus of the Penicillin gene cluster.