pSrTA1662-toolkit
(Plasmid
#154372)
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PurposeExpresses SrTA1662 in plants
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 154372 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2356
- Total vector size (bp) 12817
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Vector typeBacterial Expression, Plant Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsE.coli K12 OmniMAX™ 2 T1R bacterial strain should be used for expression
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSrTA1662
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SpeciesAegilops tauschii
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Insert Size (bp)10461
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GenBank IDMG763911.1
- Promoter Native promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 -20 (forward)
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySynthetic SrTA1662 gene was assembled by Invitrogen (ThermoFisher) from synthetic oligonucleotides and/ or PCR products.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSrTA1662-toolkit was a gift from Brande Wulff (Addgene plasmid # 154372 ; http://n2t.net/addgene:154372 ; RRID:Addgene_154372) -
For your References section:
Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement. Gaurav K, Arora S, Silva P, Sanchez-Martin J, Horsnell R, Gao L, Brar GS, Widrig V, John Raupp W, Singh N, Wu S, Kale SM, Chinoy C, Nicholson P, Quiroz-Chavez J, Simmonds J, Hayta S, Smedley MA, Harwood W, Pearce S, Gilbert D, Kangara N, Gardener C, Forner-Martinez M, Liu J, Yu G, Boden SA, Pascucci A, Ghosh S, Hafeez AN, O'Hara T, Waites J, Cheema J, Steuernagel B, Patpour M, Justesen AF, Liu S, Rudd JC, Avni R, Sharon A, Steiner B, Kirana RP, Buerstmayr H, Mehrabi AA, Nasyrova FY, Chayut N, Matny O, Steffenson BJ, Sandhu N, Chhuneja P, Lagudah E, Elkot AF, Tyrrell S, Bian X, Davey RP, Simonsen M, Schauser L, Tiwari VK, Randy Kutcher H, Hucl P, Li A, Liu DC, Mao L, Xu S, Brown-Guedira G, Faris J, Dvorak J, Luo MC, Krasileva K, Lux T, Artmeier S, Mayer KFX, Uauy C, Mascher M, Bentley AR, Keller B, Poland J, Wulff BBH. Nat Biotechnol. 2022 Mar;40(3):422-431. doi: 10.1038/s41587-021-01058-4. Epub 2021 Nov 1. 10.1038/s41587-021-01058-4 PubMed 34725503
Map uploaded by the depositor.
