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(Plasmid #154420)


Item Catalog # Description Quantity Price (USD)
Plasmid 154420 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
  • Backbone manufacturer
    Rual et al, Genome Res. 14:2128-2135, 2004.
  • Backbone size w/o insert (bp) 5005
  • Vector type
    Gateway-compatible Entry vector, with insert of ORF6 CDS with a TEV sequence at the N-term.

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    SARS-CoV-2 isolate Wuhan-Hu-1
  • Insert Size (bp)
  • Mutation
    Many synonymous changes due to codon optimization
  • Entrez Gene
    ORF6 (a.k.a. GU280_gp06)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PCR amplified

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223 SARS-CoV2 TEV-ORF6 was a gift from Fritz Roth (Addgene plasmid # 154420 ; ; RRID:Addgene_154420)
  • For your References section:

    A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP. G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. 10.1534/g3.120.401554 PubMed 32763951