pMXS-IRES-BLAST co hPHGDH
(Plasmid #154915)

• Purpose: Express codon optimized human PHGDH
• Depositing Lab: Richard Possemato
• Publication: Richard et al Richard Possemato Lab plasmids

Backbone

• Vector backbone: pMXS-IRES-blast
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 7200
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PHGDH, codon optimized
• Alt name: Phosphoglycerate dehydrogenase
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1600
• Mutation: Codon optimized for expression in human cells
• Entrez Gene: PHGDH (a.k.a. 3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: AGTAGACGGCATCGCAGCTTGGATA
• 3′ sequencing primer: CGGCCTTATTCCAAGCGGCTTCG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry