Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pET28-Strep-TEV-Nsp14
(Plasmid #154955)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 154955 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pET28
  • Backbone size w/o insert (bp) 5233
  • Total vector size (bp) 6868
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Strep-TEV-Nsp14
  • Alt name
    Non-structural protein 14
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    1635
  • GenBank ID
    MN985325
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • Strep-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was PCR amplified from the relevant plasmid described in Gordon et al, Nature (2020); doi: 10.1038/s41586-020-2286-9

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-Strep-TEV-Nsp14 was a gift from Justin Kollman (Addgene plasmid # 154955 ; http://n2t.net/addgene:154955 ; RRID:Addgene_154955)