Ad-HisFlag-USP19ER
(Plasmid #155244)

• Purpose: for generating adenoviruses expressing the ER isoform of rat USP19
• Depositing Lab: Simon Wing
• Publication: Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23. doi: 10.1091/mbc.E14-06-1129. Epub 2015 Jan 7.

Backbone

• Vector backbone: pAdEasy/pShuttle-IRES-hrGFP-1
• Backbone manufacturer: Agilent
• Backbone size w/o insert (bp): 38900
• Vector type: Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: USP19 (ER isoform)
• Alt name: GFP
• Species: R. norvegicus (rat)
• Insert Size (bp): 4074
• Mutation: H118R- please see dep. comments
• Entrez Gene: Usp19
• Promoter: CMV
• Tag / Fusion Protein:
  • 6XHis-3XFlag (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor confirms H118R mutation does not affect plasmid function.