pOD_004
(Plasmid #155361)

• Purpose: (Empty Backbone) Gentamycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella
• Depositing Lab: Elisabeth Raleigh
• Publication: Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. doi: 10.1371/journal.pgen.1009943. eCollection 2022 Apr.

Backbone

• Vector backbone: pDEL
• Backbone manufacturer: Ilya B. Tikh and James C. Samuelson1
• Backbone size (bp): 4366
• Modifications to backbone: Replacment of KanR resistance cassette by Gentamicin resistance cassette. Gentamicin cassette was amplified from TT24691 from Roth lab ( http://rothlab.ucdavis.edu/cgi-bin/textQuery.cgi )
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Growth instructions: The plasmid can be maintained in Pir2 strain.
• Copy number: Unknown

Resource Information

• Supplemental Documents:
  • pOD_004.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

- The destination strain should be recA+ and not be able to maintain R6K plasmid
- Gentamicin is used to select colonies with the integrated vector.
- The rhaBAD and lac promoters can be induced with 0.2% rhamnose and 0.5mM IPTG