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Dlx2
(Plasmid #15537)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15537 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    E61 (pBS derivative)
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dlx2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • Mutation
    Partial cDNA
  • Entrez Gene
    Dlx2 (a.k.a. AW121999, Dlx-2, Tes-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

To make antisense probe, cut with HindIII for full-length probe or EcoRI for 560 bp probe without the homeobox, and transcribe with T3 RNA polymerase. To make sense probe, cut with NotI and transcribe with T7 RNA polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dlx2 was a gift from John Rubenstein (Addgene plasmid # 15537 ; http://n2t.net/addgene:15537 ; RRID:Addgene_15537)
  • For your References section:

    Isolation and characterization of a novel cDNA clone encoding a homeodomain that is developmentally regulated in the ventral forebrain. Porteus MH, Bulfone A, Ciaranello RD, Rubenstein JL. Neuron. 1991 Aug . 7(2):221-9. 10.1016/0896-6273(91)90260-7 PubMed 1678612