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Addgene

Nkx6.2
(Plasmid #15542)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15542 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript SK-
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nkx6.2
  • Alt name
    Nkx-6.2
  • Species
    M. musculus (mouse)
  • Mutation
    Partial cDNA
  • Entrez Gene
    Nkx6-2 (a.k.a. Gtx, Nkx6.2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make antisense probe, cut with BamHI and transcribe with T7 RNA polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nkx6.2 was a gift from John Rubenstein (Addgene plasmid # 15542 ; http://n2t.net/addgene:15542 ; RRID:Addgene_15542)
  • For your References section:

    Control of anteroposterior and dorsoventral domains of Nkx-6.1 gene expression relative to other Nkx genes during vertebrate CNS development. Qiu M, Shimamura K, Sussel L, Chen S, Rubenstein JL. Mech Dev. 1998 Mar . 72(1-2):77-88. 10.1016/S0925-4773(98)00018-5 PubMed 9533954