Nkx6.2
(Plasmid
#15542)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15542 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript SK-
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNkx6.2
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Alt nameNkx-6.2
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SpeciesM. musculus (mouse)
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MutationPartial cDNA
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Entrez GeneNkx6-2 (a.k.a. Gtx, Nkx6.2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To make antisense probe, cut with BamHI and transcribe with T7 RNA polymerase.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nkx6.2 was a gift from John Rubenstein (Addgene plasmid # 15542 ; http://n2t.net/addgene:15542 ; RRID:Addgene_15542) -
For your References section:
Control of anteroposterior and dorsoventral domains of Nkx-6.1 gene expression relative to other Nkx genes during vertebrate CNS development. Qiu M, Shimamura K, Sussel L, Chen S, Rubenstein JL. Mech Dev. 1998 Mar . 72(1-2):77-88. 10.1016/S0925-4773(98)00018-5 PubMed 9533954