Sup35 CaNM K227C His7
(Plasmid #15599)

• Depositing Lab: Susan Lindquist
• Publication: Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Backbone

• Vector backbone: pAED4
• Backbone size w/o insert (bp): 3200
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sup35 NM
• Alt name: Sup35
• Species: Candida albicans
• Insert Size (bp): 900
• Mutation: K227C. Lysine 227 mutated to cysteine.
• Tag / Fusion Protein:
  • 7xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jonathan Weissman's lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protein expression inducible by IPTG. Ca = Candida albicans, NM = N-terminal and Middle domains of Sup35.

Lindquist plasmid # 8632.