pLentiCMVblast_SLC38A2_N82A
(Plasmid
#156181)
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PurposeCMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.
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Depositing Lab
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Depositing OrganizationNYU Grossman School of Medicine
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 156181 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 156181-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 156181-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepLenti CMV Blast DEST
- Backbone size w/o insert (bp) 8000
- Total vector size (bp) 9216
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSLC38A2
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Alt nameSNAT2
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SpeciesH. sapiens (human)
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Insert Size (bp)1521
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MutationSilent mutations to prevent targeting by sgRNA: TAATCTGAGCAATGCGATTG. N82A (AAT --> GCT) "transport defective" mutation to reduce sodium affinity.
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GenBank IDBC040342.1
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Entrez GeneSLC38A2 (a.k.a. ATA2, PRO1068, SAT2, SNAT2)
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 156181-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 156181-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLentiCMVblast_SLC38A2_N82A was a gift from Alec Kimmelman (Addgene plasmid # 156181 ; http://n2t.net/addgene:156181 ; RRID:Addgene_156181) -
For your References section:
Selective alanine transporter utilization creates a targetable metabolic niche in pancreatic cancer. Parker SJ, Amendola CR, Hollinshead KER, Yu Q, Yamamoto K, Encarnacion-Rosado J, Rose RE, LaRue MM, Sohn ASW, Biancur DE, Paulo JA, Gygi SP, Jones DR, Wang H, Philips MR, Bar-Sagi D, Mancias JD, Kimmelman AC. Cancer Discov. 2020 Apr 27. pii: 2159-8290.CD-19-0959. doi: 10.1158/2159-8290.CD-19-0959. 10.1158/2159-8290.CD-19-0959 PubMed 32341021
