M71-IRES-tauGFP LTNL TV
(Plasmid
#15639)
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Depositing Lab
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Depositing OrganizationRockefeller University
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15639 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 15639-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 15639-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBS
- Backbone size w/o insert (bp) 3000
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Vector typehigh copy number
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameM71-IRES-tauGFP LTNL TV
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SpeciesM. musculus (mouse)
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Insert Size (bp)16000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13
- 3′ sequencing primer reverse
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The mouse strain that was derived from this plasmid is available from The Jackson Laboratory: http://jaxmice.jax.org/strain/006676.html
The sequencing primer T496 is at the 3' end of the IRES.
DNA (Catalog # 15639-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 15639-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M71-IRES-tauGFP LTNL TV was a gift from Peter Mombaerts (Addgene plasmid # 15639 ; http://n2t.net/addgene:15639 ; RRID:Addgene_15639) -
For your References section:
A contextual model for axonal sorting into glomeruli in the mouse olfactory system. Feinstein P, Mombaerts P. Cell. 2004 Jun 11. 117(6):817-31. 10.1016/j.cell.2004.05.011 PubMed 15186781