PRAS40 (mouse) shRNA
(Plasmid #15672)

• Depositing Lab: John Lawrence
• Publication: Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-44.

Backbone

• Vector backbone: pLKO.1
• Backbone manufacturer: Available at Addgene (#8453)
• Backbone size w/o insert (bp): 7032
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRAS40 shRNA
• Alt name: Akt1S shRNA
• Alt name: PRAS40
• Species: M. musculus (mouse)
• Insert Size (bp): 59
• GenBank ID: NM_026270
• Entrez Gene: Akt1s1 (a.k.a. 1110012J22Rik, AI227026, Lobe, Lobel, PRAS40)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: pLKO.1 5'

Resource Information

• Reference:
  • Addgene's pLKO.1 protocol
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Oligonucleotides encoding the short-hairpin RNA expression cassette targeting mouse PRAS40 from base 753-773 (GI:124376717) were annealed and subcloned into pLKO.1. See author's map for shRNA sequence information.