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(Plasmid #157689)


Item Catalog # Description Quantity Price (USD)
Plasmid 157689 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
  • Backbone manufacturer
    Thermo Fisher
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Mutation
    codon optimized
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • Flag-His8 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT-TO-FH-Nsp7 was a gift from David Tollervey (Addgene plasmid # 157689 ; ; RRID:Addgene_157689)
  • For your References section:

    Integrative vectors for regulated expression of SARS-CoV-2 proteins implicated in RNA metabolism. Bresson S, Robertson N, Sani E, Turowski TW, Shchepachev V, Kompauerova M, Spanos C, Helwak A, Tollervey D. Wellcome Open Res. 2020 Nov 3;5:261. doi: 10.12688/wellcomeopenres.16322.1. eCollection 2020. 10.12688/wellcomeopenres.16322.1 PubMed 33313418