Drosophila Nedd2-like caspase (DRONC)
(Plasmid #157778)

• Purpose: E. coli expression vector pET23 with C-term 6xHis tag
• Depositing Lab: Guy Salvesen
• Publication: Snipas et al Cell Death Differ. 2008 May;15(5):938-45. doi: 10.1038/cdd.2008.23. Epub 2008 Feb 29.

Backbone

• Vector backbone: pET23b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 5066
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Drosophila Nedd2-like caspase (DRONC)
• Alt name: DRONC
• Species: D. melanogaster (fly)
• Insert Size (bp): 1400
• Entrez Gene: Dronc (a.k.a. Dmel_CG8091, CG8090, CG8091, DRONC, Dmel\CG8091, Dronc/Casp9, Nc, Nc\Dronc, anon-EST:GreesD6, anon-EST:GressD6, anon-WO0118547.313, caspase-9, dronc)
• Promoter: T7
• Tag / Fusion Protein:
  • 6x HIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7 Promotor
• 3′ sequencing primer: T7 Terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmid pET23b encoding Drosophila wt DRONC plus a C-terminal His6 purification tag was a generous gift from Dr Rollie Clem.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry