pDendra2-H2b
(Plasmid #157817)

• Purpose: Fluorescent reporter for histone H2B tracking
• Depositing Lab: Xin Chen
• Publication: Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi: 10.1016/j.celrep.2020.108003.

Backbone

• Vector backbone: Dendra2-N1
• Backbone size w/o insert (bp): 4750
• Total vector size (bp): 5087
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Histone H2B
• Alt name: Hist1h2bb
• Species: M. musculus (mouse)
• Insert Size (bp): 378
• GenBank ID: 319178
• Entrez Gene: H2bc3 (a.k.a. H2b-143, Hist1h2bb)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry